Complementary transcriptomic and metabolomics analysis reveal the molecular mechanisms of EGCG3 ‘ ‘Me biosynthesis in Camellia sinensis was written by Huang, Xiangxiang;Yu, Shuangshuang;Chen, Si;Lin, Haiyan;Luo, Yong;Li, Juan;Zhu, Mingzhi;Wang, Kunbo. And the article was included in Scientia Horticulturae (Amsterdam, Netherlands) in 2022.Computed Properties of C30H26O12 The following contents are mentioned in the article:
Epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3 ‘ ‘Me), naturally occurring in specific tea plant cultivars, is famous for its decent bioavailability and antiallergic activity. To investigate the mol. mechanisms of EGCG3 ‘ ‘Me biosynthesis and transcriptional regulation in tea leaves, we combined multi-omics data from three tea plant cultivars (Fuding Dabaicha, FD; Mingke 1 (Jinguanyin), JGY; and Jinmudan, JMD). High-performance liquid chromatog. anal. indicated that EGCG3 ‘ ‘Me content was highest in JMD (7.78 mg/g), moderate in JGY (3.12 mg/g), and lowest in FD (0 mg/g), which was similar to the results of the non-targeted metabolic anal. The high level of EGCG3 ‘ ‘Me in JMD was concomitant with high levels of monomeric and polymerized catechin derivatives, but low levels of flavonols, flavones, and their glycosides, which was consistent with the expression levels of most catechin/flavonoid biosynthetic genes. Among them, the expression level of the CCoAOMT gene in the three tea plant cultivars was pos. associated with the relative content of EGCG3 ‘ ‘Me. Further verification revealed that CCoAOMT protein could directly catalyze the formation of EGCG3 ‘ ‘Me. We further identified 38 transcription factors from transcriptome as promising regulatory candidates for regulating CCoAOMT gene expression in tea leaves. These findings provide an essential foundation for further research on the biosynthesis and transcriptional regulation of O-methylated catechins in tea leaves. This study involved multiple reactions and reactants, such as (2R,2’R,3R,3’R,4R)-2,2′-Bis(3,4-dihydroxyphenyl)-[4,8′-bichromane]-3,3′,5,5′,7,7’-hexaol (cas: 29106-49-8Computed Properties of C30H26O12).
(2R,2’R,3R,3’R,4R)-2,2′-Bis(3,4-dihydroxyphenyl)-[4,8′-bichromane]-3,3′,5,5′,7,7′-hexaol (cas: 29106-49-8) belongs to alcohols. Alcohols are weak acids. The most acidic simple alcohols (methanol and ethanol) are about as acidic as water, and most other alcohols are somewhat less acidic. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Computed Properties of C30H26O12
Referemce:
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