Combination of UPLC-Q-TOF/MS and Network Pharmacology to Reveal the Mechanism of Qizhen Decoction in the Treatment of Colon Cancer was written by Kong, Xianbin;Liu, Chuanxin;Lu, Peng;Guo, Yuzhu;Zhao, Chenchen;Yang, Yuying;Bo, Zhichao;Wang, Fangyuan;Peng, Yingying;Meng, Jingyan. And the article was included in ACS Omega in 2021.Reference of 27208-80-6 The following contents are mentioned in the article:
Traditional Chinese medicine (TCM) has been utilized for the treatment of colon cancer. Qizhen decoction (QZD), a potential compound prescription of TCM, possesses multiple biol. activities. It has been proven clin. effective in the treatment of colon cancer. However, the mol. mechanism of anticolon cancer activity is still not clear. This study aimed to identify the chem. composition of QZD. Furthermore, a collaborative anal. strategy of network pharmacol. and cell biol. was used to further explore the critical signaling pathway of QZD anticancer activity. First, ultraperformance liquid chromatog.-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) was performed to identify the chem. composition of QZD. Then, the chem. composition database of QZD was constructed based on a systematic literature search and review of chem. constituents. Moreover, the common and indirect targets of chem. components of QZD and colon cancer were searched by multiple databases. A protein-protein interaction (PPI) network was constructed using the String database (https://www.string-db.org/). All of the targets were analyzed by Gene Oncol. (GO) bioanal. and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway anal., and the visual network topol. diagram of “Prescription-TCM-Chem. composition-Direct target-Indirect target-Pathway” was constructed by Cytoscape software (v3.7.1). The top mol. pathway ranked by statistical significance was further verified by mol. biol. methods. The results of UPLC-Q-TOF/MS showed that QZD had 111 kinds of chem. components, of which 103 were unique components and 8 were common components. Ten pivotal targets of QZD in the treatment of colon cancer were screened by the PPI network. Targets of QZD involve many biol. processes, such as the signaling pathway, immune system, gene expression, and so on. QZD may interfere with biol. pathways such as cell replication, oxygen-containing compounds, or organic matter by protein binding, regulation of signal receptors or enzyme binding, and affect cytoplasm and membrane-bound organelles. The main antitumor core pathways were the apoptosis metabolic pathway, the PI3K-Akt signal pathway, and so on. Expression of the PI3K-Akt signal pathway was significantly downregulated after the intervention of QZD, which was closely related to the inhibition of proliferation and migration of colon cancer cells by cell biol. methods. The present work may facilitate a better understanding of the effective components, therapeutic targets, biol. processes, and signaling pathways of QZD in the treatment of colon cancer and provide useful information about the utilization of QZD. This study involved multiple reactions and reactants, such as (2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 27208-80-6Reference of 27208-80-6).
(2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 27208-80-6) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. Secondary alcohols are easily oxidized without breaking carbon-carbon bonds only as far as the ketone stage. No further oxidation is seen except under very stringent conditions.Reference of 27208-80-6
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