An improved analytical method for determination of trans-resveratrol and related stilbenes in grape skin by QuEChERS coupled with HPLC-PDA-MS was written by Hua, Leng-Huei;Stephen Inbaraj, Baskaran;Chen, Bing-Huei. And the article was included in International Journal of Food Science and Technology in 2021.Application In Synthesis of (2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol The following contents are mentioned in the article:
Grape is a popular fruit crop that generates about one-fourth of total weight as pomace during winemaking and com. juice production However, a significant amount of resveratrol and related stilbenes is present in grape skin, and their effective isolation enables the production of functional foods. Herein, an improved anal. method for the determination of trans-resveratrol and related stilbenes in grape skin waste by QuEChERS method coupled with a high-performance liquid chromatograph-photodiode array detector-mass spectrometer was developed. By employing a Gemini C18 column and a mobile phase of acetic acid in water (pH 3.6) and acetonitrile, a total of five resveratrol and related stilbenes could be seperated within 15 min with a flow rate of 0.8 mL min-1 and detection wavelength at 306 nm. The optimized QuEChERS conditions were 10 mL of acetonitrile, 1 mL of grape skin extract and 900 mg of magnesium sulfate plus 25 mg of primary secondary amine. Principal component anal. by two principal components (74.18%, 25.82%) could well describe the trans-resveratrol recovery at different QuEChERS conditions. A mean recovery, as well as coefficient of variation (CV, %) of repeatability and intermediate precision, ranged from 96.6% to 104.4%, 1.96% to 3.10% and 1.68% to 4.27%, resp., conforming to the regulation set by Taiwan Food and Drug Administration. Quantitation revealed cis-piceid to be present in largest amount (2.616μg mL-1), followed by trans-piceid (1.027μg mL-1), trans-resveratrol (0.967μg mL-1), ε-viniferin (0.660μg mL-1) and cis-resveratrol (0.183μg mL-1) in grape skin extract, with their corresponding contents in dried grape skin being 4.185, 1.643, 1.539, 1.056 and 0.293μg g-1, resp. This study involved multiple reactions and reactants, such as (2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 27208-80-6Application In Synthesis of (2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol).
(2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (cas: 27208-80-6) belongs to alcohols. Similar to water, an alcohol can be pictured as having an sp3 hybridized tetrahedral oxygen atom with nonbonding pairs of electrons occupying two of the four sp3 hybrid orbitals. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.Application In Synthesis of (2S,3R,4S,5S,6R)-2-(3-Hydroxy-5-((E)-4-hydroxystyryl)phenoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
Referemce:
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