SDS of cas: 627-18-9In 2021 ,《Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay》 appeared in ChemBioChem. The author of the article were Klaus, Miriam; Zurek, Paul Jannis; Kaminski, Tomasz S.; Pushpanath, Ahir; Neufeld, Katharina; Hollfelder, Florian. The article conveys some information:
The exploration of large DNA libraries of metagenomic or synthetic origin is greatly facilitated by ultrahigh-throughput assays that use monodisperse water-in-oil emulsion droplets as sequestered reaction compartments. Millions of samples can be generated and analyzed in microfluidic devices at kHz speeds, requiring only micrograms of reagents. The scope of this powerful platform for the discovery of new sequence space is, however, hampered by the limited availability of assay substrates, restricting the functions and reaction types that can be investigated. Here, we broaden the scope of detectable biochem. transformations in droplet microfluidics by introducing the first fluorogenic assay for alc. dehydrogenases (ADHs) in this format. We have synthesized substrates that release a pyranine fluorophore (8-hydroxy-1,3,6-pyrenetrisulfonic acid, HPTS) when enzymic turnover occurs. Pyranine is well retained in droplets for >6 wk (i. e. 14-times longer than fluorescein), avoiding product leakage and ensuring excellent assay sensitivity. Product concentrations as low as 100 nM were successfully detected, corresponding to less than one turnover per enzyme mol. on average The potential of our substrate design was demonstrated by efficient recovery of a bona fide ADH with an >800-fold enrichment. The repertoire of droplet screening is enlarged by this sensitive and direct fluorogenic assay to identify dehydrogenases for biocatalytic applications. The experimental process involved the reaction of 3-Bromopropan-1-ol(cas: 627-18-9SDS of cas: 627-18-9)
3-Bromopropan-1-ol(cas: 627-18-9) is used in the synthesis of fluorescent halide-sensitive quinolinium dyes, chiral, quaternary prolines through cyclization of quaternary amino acids and molten salt-polymers. It is utilized for the study of micellar media and in microemulsions based on cationic or a nonionic surfactant by reacting with phenols.SDS of cas: 627-18-9
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts