The author of ãDesign, synthesis and in vitro evaluation of coumarin-imidazo[1,2-a]pyridine derivatives against cancer induced osteoporosisã?were Sashidhara, Koneni V.; Singh, L. Ravithej; Choudhary, Dharmendra; Arun, Ashutosh; Gupta, Sampa; Adhikary, Sulekha; Palnati, Gopala Reddy; Konwar, Rituraj; Trivedi, Ritu. And the article was published in RSC Advances in . Reference of 5-(tert-Butyl)-4-hydroxyisophthalaldehyde The author mentioned the following in the article:
A series of biol. important 6-(imidazo[1,2-a]pyridin-2-yl)-2H-chromen-2-one derivatives were synthesized by employing the silver(I) catalyzed Groebke-Blackburn-Bienayme multicomponent reaction. The synthesized compounds were tested in a primary calvarial osteoblast cells by alk. phosphatase assay and an alizarin red-S staining assay for their possible osteoprotective properties. Further, the effects of active compounds 6h, 6l, and 6o on the expression of osteogenic genes BMP2, RUNX2, COL1, and OCN were measured by qPCR. Out of three promising compounds, 6l and 6o significantly induced apoptosis in MDA-MB-231 cancer cells via mitochondrial depolarisation without affecting normal cells. In an in vitro co-culture model of bone metastasis, we investigated the ability of coumarin-imidazo[1,2-a]pyridine hybrids to reverse the neg. impact of MDA-MB-231 cancer cells on osteoblast differentiation. The results illustrate the potential of designed hybrids to re-establish the bone homeostasis. These findings demonstrate the significance of newly synthesized hybrids as lead mols., possessing both antiosteoporotic and anticancer properties that can be developed into new therapeutic agents to alleviate osteoporosis and bone metastasis. In the experimental materials used by the author, we found 5-(tert-Butyl)-4-hydroxyisophthalaldehyde(cas: 153759-59-2Reference of 5-(tert-Butyl)-4-hydroxyisophthalaldehyde)
5-(tert-Butyl)-4-hydroxyisophthalaldehyde(cas: 153759-59-2) belongs to phenols.Deprotonation of a phenol forms a corresponding negative phenolate ion or phenoxide ion, and the corresponding salts are called phenolates or phenoxides (aryloxides according to the IUPAC Gold Book).Reference of 5-(tert-Butyl)-4-hydroxyisophthalaldehyde
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