Rakauskaite, Rasa; Urbanaviciute, Giedre; Simanavicius, Martynas; Lasickiene, Rita; Vaitiekaite, Ausra; Petraityte, Grazina; Masevicius, Viktoras; Zvirbliene, Aurelija; Klimasauskas, Saulius published their research in iScience on December 18 ,2020. The article was titled ãPhotocage-Selective Capture and Light-Controlled Release of Target Proteinsã?Synthetic Route of C8H9NO5S The article contains the following contents:
Photochem. transformations enable exquisite spatiotemporal control over biochem. processes; however, methods for reliable manipulations of biomols. tagged with biocompatible photo-sensitive reporters are lacking. Here we created a high-affinity binder specific to a photolytically removable caging group. We utilized chem. modification or genetically encoded incorporation of noncanonical amino acids to produce proteins with photocaged cysteine or selenocysteine residues, which were used for raising a high-affinity monoclonal antibody against a small photoremovable tag, 4,5-dimethoxy-2-nitrobenzyl (DMNB) group. Employing the produced photocage-selective binder, we demonstrate selective detection and immunoprecipitation of a variety of DMNB-caged target proteins in complex biol. mixtures This combined orthogonal strategy permits photocage-selective capture and light-controlled traceless release of target proteins for a myriad of applications in nanoscale assays. After reading the article, we found that the author used 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate(cas: 76931-93-6Synthetic Route of C8H9NO5S)
2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate(cas: 76931-93-6) belongs to pyrrolidine. Pyrrolidine being a good nucleophile easily undergoes electrophilic substitution reactions with different electrophiles such alkyl halides and acyl halides, and forms N-substituted pyrrolidines. N-Alkylpyrrolidine on further reaction with alkyl halide provided quaternary salts.Synthetic Route of C8H9NO5S
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