In 2018,Schoonen, Lise; Eising, Selma; van Eldijk, Mark B.; Bresseleers, Jaleesa; van der Pijl, Margo; Nolte, Roeland J. M.; Bonger, Kimberly M.; van Hest, Jan C. M. published 《Modular, Bioorthogonal Strategy for the Controlled Loading of Cargo into a Protein Nanocage》.Bioconjugate Chemistry published the findings.Synthetic Route of C7H9NO2 The information in the text is summarized as follows:
Virus capsids, i.e., viruses devoid of their genetic material, are suitable nanocarriers for biomedical applications such as drug delivery and diagnostic imaging. For this purpose, the reliable encapsulation of cargo in such a protein nanocage is crucial, which can be accomplished by the covalent attachment of the compounds of interest to the protein domains positioned at the interior of the cage. This approach is particularly valid for the capsid proteins of the cowpea chlorotic mottle virus (CCMV), which have their N-termini located at the inside of the capsid structure. Here, the authors examined several site-selective modification methods for covalent attachment and encapsulation of cargo at the N-terminus of the CCMV protein. Initially, the authors explored approaches to introduce an N-terminal azide functionality, which would allow the subsequent bioorthogonal modification with a strained alkyne to attach the desired cargo. As these methods showed compatibility issues with the CCMV capsid proteins, a strategy based on 2-pyridinecarboxaldehydes for site-specific N-terminal protein modification was employed. This method allowed the successful modification of the proteins, and was applied for the introduction of a bioorthogonal vinylboronic acid moiety. In a subsequent reaction, the proteins could be modified further with a fluorophore using the tetrazine ligation. The application of capsid assembly conditions on the functionalized proteins led to successful particle formation, showing the potential of this covalent encapsulation strategy. In the part of experimental materials, we found many familiar compounds, such as 2,6-Pyridinedimethanol(cas: 1195-59-1Synthetic Route of C7H9NO2)
2,6-Pyridinedimethanol(cas: 1195-59-1) belongs to pyridine. Pyridine’s structure is isoelectronic with that of benzene, but its properties are quite different. Pyridine is completely miscible with water, whereas benzene is only slightly soluble. Like all hydrocarbons, benzene is neutral (in the acid–base sense), but because of its nitrogen atom, pyridine is a weak base.Synthetic Route of C7H9NO2
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts