《Light-induced enzyme deactivation as a new tool to prevent cross reactions in multistep enzyme cascades》 was published in Chemie Ingenieur Technik in 2020. These research results belong to Gerlach, T.; Drepper, T.; Rother, D.. Quality Control of 3-Hydroxybenzaldehyde The article mentions the following:
To eliminate cross reactivity in one-pot reaction systems, enzymes prone to side reactivities are combined with produce reactive oxygen species upon irradiation The enzyme fusions can be added to the corresponding reaction step enabling the catalyst activity to be switched off selectively. Here, an enzyme cascade starting from 3-hydroxy benzaldehyde and pyruvate to a trisubstituted tetrahydroisoquinoline has been chosen as a test system. CA> The first cascade step encompasses a carboligation conducted by the pyruvate decarboxylase variant E469G/W543H from Acetobacter pasteurianus (ApPDC E469G/W54m). Afterwards a reductive amination occurs, mediated by the Chromobacterium violaceum transaminase (Cv2025). This cascade is a suitable target as in a one-pot reaction approach across reactivity occurs. The ApPDC E469G/W543H was genetically linked to a photosensitizer, the singlet oxygen photosensitizing protein (SOPP3). After illumination with blue light (450 nm), the relative activity of SOPP3-ApPDC E469G/W543H could be reducedto 10% (Fig. A). The Cv2025 was inactivated simply by illuminating the cofactor of the transaminase pyridoxal 5-phosphate and could also be inactivated to 10% residual activity (Fig.B). As both inactivation, steps still need 30-120 min to reach the goal, the light-inactivation setups are current target to optimization. The experimental process involved the reaction of 3-Hydroxybenzaldehyde(cas: 100-83-4Quality Control of 3-Hydroxybenzaldehyde)
3-Hydroxybenzaldehyde(cas: 100-83-4) can be used as a reactant along with ethyl acetoacetate and thiourea in the synthesis of corresponding dihydropyrimidine-2-thione (monastrol), using Yb(OTf)3 as a catalyst by Biginelli cyclocondensation reaction.Quality Control of 3-Hydroxybenzaldehyde
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts