Mattiassi, Sabrina; Rizwan, Muhammad; Grigsby, Christopher L.; Zaw, Aung Moe; Leong, Kam W.; Yim, Evelyn K. F. published an article in 2021. The article was titled 《Enhanced efficiency of nonviral direct neuronal reprogramming on topographical patterns》, and you may find the article in Biomaterials Science.Name: 4-Aminobutan-1-ol The information in the text is summarized as follows:
Nonviral direct neuronal reprogramming holds significant potential in the fields of tissue engineering and regenerative medicine. However, the issue of low reprogramming efficiency poses a major barrier to its application. We propose that topog. cues, which have been applied successfully to enhance lineage-directed differentiation and multipotent stem cell transdifferentiation, could improve nonviral direct neuronal reprogramming efficiency. To investigate, we used a polymer-BAM (Brn2, Ascl1, Myt1l) factor transfection polypex to reprogram primary mouse embryonic fibroblasts. Using a multiarchitecture chip, we screened for patterns that may improve transfection and/or subsequent induced neuron reprogramming efficiency. Selected patterns were then investigated further by analyzing β-tubulin III (TUJ1) and microtubule-associated protein 2 (MAP2) protein expression, cell morphol. and electrophysiol. function of induced neurons. Certain hierarchical topogs., with nanopatterns imprinted on micropatterns, significantly improved the percentage of TUJ1+ and MAP2+ cells. It is postulated that the microscale base pattern enhances initial BAM expression while the nanoscale sub-pattern promotes subsequent maturation. This is because the base pattern alone increased expression of TUJ1 and MAP2, while the nanoscale pattern was the only pattern yielding induced neurons capable of firing multiple action potentials. Nanoscale patterns also produced the highest fraction of cells showing spontaneous synaptic activity. Overall, reprogramming efficiency with one dose of polyplex on hierarchical patterns was comparable to that of five doses without topog. Thus, topog. can enhance nonviral direct reprogramming of fibroblasts into induced neurons. The experimental process involved the reaction of 4-Aminobutan-1-ol(cas: 13325-10-5Name: 4-Aminobutan-1-ol)
4-Aminobutan-1-ol(cas: 13325-10-5) is used in the synthesis of NSAIDs with anti-inflammatory properties. Also used in the synthesis of polyamine transport ligands with specificity against human cancers allowing easy access to specific cancer cells.Name: 4-Aminobutan-1-ol
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