On August 21, 2020, Forneris, Clarissa C.; Nguy, Andy K. L.; Seyedsayamdost, Mohammad R. published an article.Quality Control of H-Phg(4-OH)-OH The title of the article was Mapping and Exploiting the Promiscuity of OxyB toward the Biocatalytic Production of Vancomycin Aglycone Variants. And the article contained the following:
Vancomycin is one of the most important clin. antibiotics in the fight against infectious disease. Its biol. activity relies on three aromatic cross-links, which create a cup-shaped topol. and allow tight binding to nascent peptidoglycan chains. The cytochrome P 450 enzymes OxyB, OxyA, and OxyC have been shown to introduce these synthetically challenging aromatic linkages. The ability to utilize the P 450 enzymes in a chemo-enzymic scheme to generate vancomycin derivatives is appealing but requires a thorough understanding of their reactivities and mechanisms. Herein, we systematically explore the scope of OxyB biocatalysis and report installation of diverse diaryl ether and biaryl cross-links with varying macrocycle sizes and compositions, when the enzyme is presented with modified vancomycin precursor peptides. The structures of the resulting products were determined using one-dimensional/two-dimensional NMR spectroscopy, high-resolution mass spectrometry (HR-MS), tandem HR-MS, and isotopic labeling, as well as UV-visible light absorption and fluorescence emission spectroscopies. An exploration of the biol. activities of these alternative OxyB products surprisingly revealed antifungal properties. Taking advantage of the promiscuity of OxyB, we chemo-enzymically generated a vancomycin aglycon variant containing an expanded macrocycle. Mechanistic implications for OxyB and future directions for creating vancomycin analog libraries are discussed. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Quality Control of H-Phg(4-OH)-OH
The Article related to oxyb monooxygenase vancomycin aglycon variant, biosynthesis, chemo-enzymatic synthesis, macrocycles, vancomycin, Placeholder for records without volume info and other aspects.Quality Control of H-Phg(4-OH)-OH
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