On September 30, 2022, Carla Guimaraes Sobrinho, Alessandra; Silva Corpes, Rosana; Isabel Portilho dos Santos, Kyouk; Maria Menezes Barra, Ivoneide; Kiyoshi Miyagawa, Helder; Silva Santos, Alberdan published an article.Formula: C3H6O4 The title of the article was Untargeted GC-MS Metabolomics applied to wild leaves and callus produced by plant tissue culture of Hibiscus sabdariffa L.. And the article contained the following:
Hibiscus sabdariffa L. is a naturalized medicinal species in Brazil commonly called a “vinagreira” and is a member of the Malvaceae Juss. family, which has a rich potential of bioactive compounds presenting extracts with antioxidant, antibacterial, anti-inflammatory, hepatoprotective, antiviral, antidiabetic, and antiobesity, among others. The production of secondary metabolites of medicinal plants using biotechnol. tools such as the culture of callus of plant tissues is increasingly being used to produce high-quality compounds under in vitro conditions. From this perspective, the objective of this work was to analyze the chem. compounds of the leaves and callus culture of H. sabdariffa using techniques of Gas Chromatog. Coupled to the Mass Spectrum (GC-MS),. The anal. methodol. used consisted of removal of liposol. compounds, acid hydrolysis, and derivatization, all stages were submitted to ultrasonic-assisted agitation, using a reduced amount of biomass. Based on the results obtained in the study, a total of 38 metabolites identified by GC-MS anal. can be observed Among the identified substances, protocatechuic acid (26A) stands out as the main constituent, with a relative abundance of 26.86% and 16.68% for leaves and callus of H. sabdariffa, resp. The principal component anal. (PCA) allowed the discrimination of the chem. composition of each sample, being useful for the observation and detection of the compounds trends patterns. The anal. of the hierarchical group combined with the heat map represented the visual relationship between the samples of the data set indicating the values of higher and lower concentrations of chem. compounds resp., confirming that protocatechuic acid is the most abundant, for the leaves and callus of H. sabdariffa, followed by eicosanoid and isocitric acid, produced only in callus. It was concluded that the GC-MS technique combined with chemometric tools, helped identify the diversity of the compounds present in the leaves and callus of H. sabdariffa and that callus culture enables the production of bioactive compounds continuously and uniformly in a controlled environment and free of contamination. The experimental process involved the reaction of 2,3-Dihydroxypropanoic acid(cas: 473-81-4).Formula: C3H6O4
The Article related to hibiscus leaf callus tissue culture gcms metabolomics, Pharmaceuticals: Pharmacognostic Products and other aspects.Formula: C3H6O4
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