Yang, Hao; Wang, Zhenfei; Shi, Shujun; Yu, Qin; Liu, Meiling; Zhang, Zhelin published an article in 2022, the title of the article was Identification of cerebrospinal fluid metabolites as biomarkers for neurobrucellosis by liquid chromatography-mass spectrometry approach.Application of 32462-30-9 And the article contains the following content:
Neurobrucellosis is the most morbid form in brucellosis disease. Metabolomics is an emerging method which intends to explore the global alterations of various metabolites in samples. We aimed to identify metabolites in cerebrospinal fluid (CSF) as biomarkers that were potentially unique for neurobrucellosis. CSF samples from 25 neurobrucellosis patients and 25 normal controls (uninfected patients with hydrocephalus) were collected for metabolite detection using liquid chromatog.-mass spectrometry (LC-MS) approach. Inflammatory cytokines in CSF were measured with ELISA (ELISA). The base peak chromatogram in CSF samples showed that small-mol. metabolites were well separated Principal Component Anal. (PCA) anal. exhibited the examined samples were arranged in two main clusters in accordance with their group. Projection to Latent Structures Discriminant Anal. (PLS-DA) revealed there was a noticeable separation between neurobrucellosis and normal groups. Orthogonal Partial Least-Squares-Discriminant Anal. (OPLS-DA) could responsibly illuminate the differences between neurobrucellosis and normal controls. Neurobrucellosis showed a total of 155 differentiated metabolites. Prominent potential biomarkers including 30 metabolites were then selected out, regarded as more capable of distinguishing neurobrucellosis. TNF-α and IL-6 in CSF were remarkably increased in neurobrucellosis. We presented the heatmaps and correlation analyses among the identified 30 potential biomarkers. In conclusion, this study showed that CSF metabolomics based on LC-MS could distinguish neurobrucellosis patients from normal controls. Our data offered perspectives for diagnosis and treatment for neurobrucellosis. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).Application of 32462-30-9
The Article related to neurobrucellosis cerebrospinal fluid metabolite biomarker lcms, neurobrucellosis, cerebrospinal fluid, liquid chromatography-mass spectrometry, metabolomics, Placeholder for records without volume info and other aspects.Application of 32462-30-9
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