Buszewska-Forajta, Magdalena; Raczak-Gutknecht, Joanna; Struck-Lewicka, Wiktoria; Niziol, Magdalena; Artymowicz, Malgorzata; Markuszewski, Marcin; Kordalewska, Marta; Matuszewski, Marcin; Markuszewsk, Michal J. published an article in 2022, the title of the article was Untargeted metabolomics study of three matrices: seminal fluid, urine, and serum to search the potential indicators of prostate cancer.Recommanded Product: 2,3-Dihydroxypropanoic acid And the article contains the following content:
The simultaneous determination of metabolites from biol. fluids may provide more accurate information about the current body condition. So far, the metabolomics approach has been successfully applied to study the mechanism of several disorders and to search for novel biomarkers. Urine and plasma are widely accepted matrixes for the evaluation of several pathologies, while prostate cancer (CaP) development is still unknown. For this reason, an alternative matrix, the seminal fluid, was proposed to expand the knowledge about the CaP pathomechanism. The main aim of this study was to develop and optimize the sample preparation protocol to ensure the highest coverage of the metabolome of ejaculate samples. Parameters like the type and composition of the solvent mixture, time of extraction, and applied volume of the solvent were tested. The optimized method was applied for the untargeted metabolomics profiling of seminal fluid samples obtained from CaP patients. Moreover, urine and serum samples were also prepared for untargeted metabolomics anal. Analyses were carried out with the use of two complementary anal. techniques: GC-EI-QqQ/MS and LC-ESI-TOF/MS. Finally, the metabolic signature of seminal fluid (n = 7), urine (n = 7), and plasma (n = 7) samples was compared. Furthermore, the hypothesis of the increased level of metabolites in ejaculate samples related to the CaP development was evaluated. The results indicated that the developed and optimized sample preparation protocol for seminal fluid may be successfully applied for metabolomics study. Untargeted anal. of ejaculate enabled to determine the following classes of compounds: fatty acids, sphingolipids, phospholipids, sugars, and their derivatives, as well as amino acids. Finally, a comparison of the three tested matrixes was carried out. To our best knowledge, it is the first time when the metabolic profile of the three matrixes, namely, urine, plasma, and seminal fluid, was compared. Based on the results, it can be pointed out that ejaculate comprises the metabolic signature of both matrixes (polar compounds characteristic for urine, and nonpolar ones present in plasma samples). Compared to plasma, semen samples revealed to have a similar profile; however, determined levels of metabolites were lower in case of ejaculate. In case of urine samples, compared to semen metabolic profiles, the levels of detected metabolites were decreased in the latter ones. The experimental process involved the reaction of 2,3-Dihydroxypropanoic acid(cas: 473-81-4).Recommanded Product: 2,3-Dihydroxypropanoic acid
The Article related to seminal fluid urine serum prostate cancer metabolomic analysis, hplc, metabolites, metabolomics, plasma, prostate cancer, seminal fluid, urine, Placeholder for records without volume info and other aspects.Recommanded Product: 2,3-Dihydroxypropanoic acid
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