Stoller, Sven; Sicoli, Giuseppe; Baranova, Tatiana Y.; Bennati, Marina; Diederichsen, Ulf published an article in 2011, the title of the article was TOPP-A Novel Nitroxide-Labeled Amino Acid for EPR Distance Measurements.COA of Formula: C8H9NO3 And the article contains the following content:
We report the synthesis of a novel, rigid nitroxide-labeled amino acid 4-(3,3,5,5-tetramethyl-2,6-dioxo-4-oxylpiperazin-1-yl)-L-phenylglycine (TOPP, I) that does not produce perturbation of the secondary structure, thus, providing a promising tool for structural studies of peptides and proteins. The design of the TOPP amino acid is based on the alignment of the nitroxide with the Cα-Cβ amino acid bond on one axis and the synthetic applicability with respect to racemization at Cα during amino acid and peptide oligomer synthesis. The present study illustrates the straightforward assignment of a spin-spin distance measured by pulsed EPR which is in contrast to the different and ambiguous result obtained with the commonly used MTSSL label. Furthermore, the predicted reduced mobility of the TOPP spin label represents a potential advantage for its incorporation into transmembrane peptides for the structure determination of peptide arrangements at an at. scale. The experimental process involved the reaction of H-Phg(4-OH)-OH(cas: 32462-30-9).COA of Formula: C8H9NO3
The Article related to topp nitroxide amino acid preparation epr distance peptide, tetramethyldioxooxylpiperazinyl phenylglycine preparation epr distance peptide, nitroxide labeled phenylglycine preparation determination distance peptide epr and other aspects.COA of Formula: C8H9NO3
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts