Zhang, Yaoyao; Wang, Jin; Cao, Xianshuang; Liu, Wei; Yu, Hongwei; Ye, Lidan published the artcile< High-level production of linalool by engineered Saccharomyces cerevisiae harboring dual mevalonate pathways in mitochondria and cytoplasm>, Recommanded Product: 3,7-Dimethylocta-1,6-dien-3-ol, the main research area is ERG20 mutant; Linalool; MVA pathway; Pathway compartmentation; Saccharomyces cerevisiae.
Linalool, a valuable monoterpene alc., is widely used in cosmetics and flavoring ingredients. However, its scalable production by microbial fermentation is not yet achieved. In this work, considerable increase in linalool production was obtained in Saccharomyces cerevisiae by dual metabolic engineering of the mevalonic acid (MVA) pathway in both mitochondria and cytoplasm. A farnesyl pyrophosphate synthase mutant ERG20F96W/N127W and a linalool synthase from Cinnamomum osmophloeum (CoLIS) were introduced and meanwhile the endogenous ERG20 was down-regulated to prevent the competitive loss of precursor. In addition, overexpression of the proteins of CoLIS and ERG20F96W/N127W and another copy of the same enzymes CoLIS/ERG20F96W/N127W with mitochondrial localization signal (MLS) were carried out to further pull the flux to linalool. Finally, a maximum linalool titer of 23.45 mg/L was obtained in a batch fermentation with sucrose as carbon source. This combinatorial engineering strategy may provide hints for biosynthesis of other monoterpenes.
Enzyme and Microbial Technology published new progress about 78-70-6. 78-70-6 belongs to class alcohols-buliding-blocks, and the molecular formula is C10H18O, Recommanded Product: 3,7-Dimethylocta-1,6-dien-3-ol.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts