Su, Hui-Hui; Peng, Fei; Ou, Xiao-Yang; Zeng, Ying-Jie; Zong, Min-Hua; Lou, Wen-Yong published the artcile< Combinatorial synthetic pathway fine-tuning and cofactor regeneration for metabolic engineering of Escherichia coli significantly improve production of D-glucaric acid>, Product Details of C6H10O8, the main research area is metabolic engineering Escherichia coli glucaric acid; Microbial fermentation; Platform chemical; RBS optimization; Self-sufficient; Systematic metabolic engineering.
D-glucaric acid (GA) has been identified as among promising biotechnol. alternatives to oil-based chems. GA and its derivatives are widely used in food additives, dietary supplements, drugs, detergents, corrosion inhibitors and biodegradable materials. The increasing availability of a GA market is improving the cost-effectiveness and efficiency of various biosynthetic pathways. In this study, an engineered Escherichia coli strain GA10 was constructed by systematic metabolic engineering. This involved redirecting metabolic flux into the GA biosynthetic pathways, blocking the conversion pathways of D-glucuronic acid (GlcA) and GA into byproducts, introducing an in situ NAD+ regeneration system and fine-tuning the activity of the key enzyme, myo-inositol oxygenase (Miox). Subsequently, the culture medium was optimized to achieve the best performance of the GA10 strain. GA was produced at 5.35 g/L (extracellular and intracellular), with a maximized yield of ∼0.46 mol/mol on D-glucose and glycerol, by batch fermentation This work demonstrates efficient biosynthetic pathways of GA in E. coli by metabolic engineering and should accelerate the application of GA biosynthetic pathways in industrial processes.
New Biotechnology published new progress about Escherichia coli. 87-73-0 belongs to class alcohols-buliding-blocks, and the molecular formula is C6H10O8, Product Details of C6H10O8.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts