Santiago, Marina; Sachdev, Shivani; Arnold, Jonathon C.; McGregor, Iain S.; Connor, Mark published the artcile< Absence of entourage: terpenoids commonly found in Cannabis sativa do not modulate the functional activity of Δ9-THC at human CB1 and CB2 receptors>, SDS of cas: 78-70-6, the main research area is Cannabis terpenoids tetrahydrocannabinol CB1 CB2 receptor; THC; cannabinoid receptor; entourage effect; phytocannabinoid; signaling; terpenoid.
We examined six common terpenoids alone, and in combination with cannabinoid receptor agonists, on CB1 and CB2 signaling in vitro. Potassium channel activity in AtT20 FlpIn cells transfected with human CB1 or CB2 receptors was measured in real time using FLIPR membrane potential dye in a FlexStation 3 plate reader. Terpenoids were tested individually and in combination for periods up to 30 min. Endogenous somatostatin receptors served as a control for direct effects of drugs on potassium channels. Alpha-Pinene, β-pinene, β-caryophyllene, linalool, limonene and β-myrcene did not change membrane potential in AtT20 cells expressing CB1 or CB2, or affect the response to a maximally effective concentration of the synthetic cannabinoid CP55,940. The presence of individual or combination of terpenoids did not affect the hyperpolarization produced by Δ9-THC. To investigate possible effect on desensitization of CB1 responses, all six terpenoids were added together with Δ9-THC and signaling measured continuously over 30 min. Terpenoids did not affect desensitization, after 30 min control hyperpolarization recovered by 63% -6% in the presence of terpenoids recovery was 61% -5%. None of the six of the most common terpenoids in Cannabis directly activated CB1 or CB2, or modulated the signaling of phytocannabinoid agonist Δ9-THC.
Cannabis and Cannabinoid Research published new progress about Behavior. 78-70-6 belongs to class alcohols-buliding-blocks, and the molecular formula is C10H18O, SDS of cas: 78-70-6.
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