Liu, Yu; Cen, Xuecong; Liu, Dehua; Chen, Zhen published the artcile< Metabolic engineering of Escherichia coli for high-yield production of (R)-1,3-butanediol>, Safety of (R)-Butane-1,3-diol, the main research area is metabolic engineering Escherichia butanediol fed batch fermentation; 1,3-butanediol; Escherichia coli; cofactor engineering; enzyme screening; metabolic engineering.
1,3-Butanediol (1,3-BDO) is an important C4 platform chem. widely used as a solvent in cosmetics and a key intermediate for the synthesis of fragrances, pheromones, and pharmaceuticals. The development of sustainable bioprocesses to produce enantiopure 1,3-BDO from renewable bioresources by fermentation is a promising alternative to conventional chem. routes and has aroused great interest in recent years. Although two metabolic pathways have been previously established for the biosynthesis of (R)-1,3-PDO, the reported titer and yield are too low for cost-competitive production In this study, we report the combination of different metabolic engineering strategies to improve the production of (R)-1,3-BDO by Escherichia coli, including (1) screening of key pathway enzymes; (2) increasing NADPH supply by cofactor engineering; (3) optimization of fermentation conditions to divert more flux into 1,3-BDO pathway; (4) reduction of byproducts formation by pathway engineering. With these efforts, the best engineered E. coli strain can efficiently produce (R)-1,3-BDO with a yield of 0.6 mol/mol glucose, corresponding to 60% of the theor. yield. Besides, we also showed the feasibility of aerobically producing 1,3-BDO via a new pathway using 3-hydroxybutyrate as an intermediate.
ACS Synthetic Biology published new progress about Escherichia coli. 6290-03-5 belongs to class alcohols-buliding-blocks, and the molecular formula is C4H10O2, Safety of (R)-Butane-1,3-diol.
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