Kim, Taeho; Stogios, Peter J.; Khusnutdinova, Anna N.; Nemr, Kayla; Skarina, Tatiana; Flick, Robert; Joo, Jeong Chan; Mahadevan, Radhakrishnan; Savchenko, Alexei; Yakunin, Alexander F. published the artcile< Rational engineering of 2-deoxyribose-5-phosphate aldolases for the biosynthesis of (R)-1,3-butanediol>, Name: (R)-Butane-1,3-diol, the main research area is engineering deoxyribosephosphate aldolase Bacillus crystal structure butanediol; 1,3-butanediol; 2-deoxyribose-5-phosphate aldolase (DERA); BH1352; Escherichia coli (E. coli); acetaldehyde condensation; aldo-keto reductase; biotechnology; crystal structure; protein engineering; site-directed mutagenesis.
Carbon-carbon bond formation is one of the most important reactions in biocatalysis and organic chem. In nature, aldolases catalyze the reversible stereoselective aldol addition between two carbonyl compounds, making them attractive catalysts for the synthesis of various chems. In this work, we identified several 2-deoxyribose-5-phosphate aldolases (DERAs) having acetaldehyde condensation activity, which can be used for the biosynthesis of (R)-1,3-butanediol (1,3BDO) in combination with aldo-keto reductases (AKRs). Enzymic screening of 20 purified DERAs revealed the presence of significant acetaldehyde condensation activity in 12 of the enzymes, with the highest activities in BH1352 from Bacillus halodurans, TM1559 from Thermotoga maritima, and DeoC from Escherichia coli. The crystal structures of BH1352 and TM1559 at 1.40-2.50 Å resolution are the first full-length DERA structures revealing the presence of the C-terminal Tyr (Tyr224 in BH1352). The results from structure-based site-directed mutagenesis of BH1352 indicated a key role for the catalytic Lys155 and other active-site residues in the 2-deoxyribose-5-phosphate cleavage and acetaldehyde condensation reactions. These experiments also revealed a 2.5-fold increase in acetaldehyde transformation to 1,3BDO (in combination with AKR) in the BH1352 F160Y and F160Y/M173I variants. The replacement of the WT BH1352 by the F160Y or F160Y/M173I variants in E. coli cells expressing the DERA + AKR pathway increased the production of 1,3BDO from glucose five and six times, resp. Thus, our work provides detailed insights into the mol. mechanisms of substrate selectivity and activity of DERAs and identifies two DERA variants with enhanced activity for in vitro and in vivo 1,3BDO biosynthesis.
Journal of Biological Chemistry published new progress about Bacillus halodurans (source of 2-deoxyribose-5-phosphate aldolase BH1352). 6290-03-5 belongs to class alcohols-buliding-blocks, and the molecular formula is C4H10O2, Name: (R)-Butane-1,3-diol.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts