Jelicic, Mario-Livio; Brusac, Edvin; Klaric, Daniela Amidzic; Nigovic, Biljana; Turk, Niksa; Mornar, Ana published the artcile< A chromatographic approach to development of 5-aminosalicylate/folic acid fixed-dose combinations for treatment of Crohn's disease and ulcerative colitis>, Quality Control of 6054-98-4, the main research area is chromatog aminosalicylate folic acid treatment Crohn disease ulcerative colitis.
Medication adherence is an important factor in inflammatory bowel disease therapy, which includes regular supplementation of malabsorbed vitamins. Absorption of folic acid is limited due to the damaging of the gastrointestinal tract, which can increase the chances to develop megaloblastic anemia and colorectal cancer. In this work, 5-aminosalicylates (mesalazine, balsalazide, sulfasalazine and olsalazine) and folic acid were characterized regarding their pharmacokinetic related properties (hydrophobicity, phospholipid and plasma protein binding) using the biomimetic chromatog. approach. Despite the high binding percentage of 5-aminosalicylates for human serum albumin (> 61.44%), results have shown that folic acid binding to human serum albumin protein is far greater (69.40%) compared to alpha 1-acid-glycoprotein (3.45%). Frontal anal. and zonal elution studies were conducted to provide an insight into the binding of folic acid to human serum albumin and potential competition with 5-aminosalicylates. The anal. method for the simultaneous determination of assay in proposed fixed-dose combinations was developed and validated according to ICH Q2 (R1) and FDA method validation guidelines. Separation of all compounds was achieved within 16 min with satisfactory resolution (Rs > 3.67) using the XBridge Ph column (150 x 4.6 mm, 3.5μm). High linearity (r > 0.9997) and precision (RSD < 2.29%) was obtained, while all recoveries were within the regulatory defined range by British (100.0 ± 5.0%) and USP (100.0 ± 10.0%). Scientific Reports published new progress about Absorption. 6054-98-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C14H8N2Na2O6, Quality Control of 6054-98-4.
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