Carr, Georgina; Haslam, Iain S.; Simmons, Nicholas L. published the artcile< Voltage Dependence of Transepithelial Guanidine Permeation Across Caco-2 Epithelia Allows Determination of the Paracellular Flux Component>, COA of Formula: C7H18ClNO5, the main research area is guanidine organic cation paracellular permeation intestine Caco2 cell.
The aim of this study was to investigate transepithelial ionic permeation via the paracellular pathway of human Caco-2 epithelial monolayers and its contribution to absorption of the base guanidine. Confluent monolayers of Caco-2 epithelial cells were mounted in Ussing chambers and the transepithelial conductance and elec. p.d. determined after NaCl dilution or medium Na substitution (bi-ionic conditions). Guanidine absorption (Ja-b) was measured ± transepithelial potential gradients using bi-ionic p.d.’s. Basal NaCl replacement with mannitol gives a transepithelial dilution p.d. of 28.0 ± 3.1 mV basal solution electropos. (PCl/PNa = 0.34). Bi-ionic p.d.’s (basal replacements) indicate a cation selectivity of NH4+ > K+∼Cs+ > Na+ > Li+ > tetraethylammonium+ > N-methyl-d-glucamine+∼choline+. Transepithelial conductances show good correspondence with bi-ionic potential data. Guanidine Ja-b was markedly sensitive to imposed transepithelial p.d. The ratio of guanidine to mannitol permeability (measured simultaneously) increased from 3.6 in the absence of an imposed p.d. to 13.8 (basolateral neg. p.d.). Hydrated monovalent ions preferentially permeate the paracellular pathway (Eisenman sequence 2 or 3). Guanidine may access the paracellular pathway because absorptive flux is sensitive to the transepithelial p.d. An alternative method to assess paracellular-mediated flux of charged organic mols. is suggested.
Pharmaceutical Research published new progress about Biological permeation (transepithelial ionic). 35564-86-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C7H18ClNO5, COA of Formula: C7H18ClNO5.
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