Bae, Kideog; Zheng, Wei; Ma, Ying; Huang, Zhiwei published the artcile< Real-Time Monitoring of Pharmacokinetics of Mitochondria-Targeting Molecules in Live Cells with Bioorthogonal Hyperspectral Stimulated Raman Scattering Microscopy>, HPLC of Formula: 10602-04-7, the main research area is raman scattering microscopy mitochondria pharmacokinetics live cells.
The dynamics of mitochondria in live cells play a pivotal role in biol. events such as cell metabolism, early stage apoptosis, and cell differentiation. Triphenylphosphonium (TPP) is a commonly used mitochondria-targeting agent for mitochondrial studies. However, there has been a lack of understanding in intracellular behaviors of TPP in the course of targeting mitochondria due to the difficulty in tracking and quantifying small mols. in a biol. environment. Here, we report the utility of hyperspectral stimulated Raman scattering (SRS) microscopy associated with a Raman tag synthesized for real-time visualization and quantitation of TPP dynamics within live cells at the subcellular level. With the myriad of merits offered by a synthesized aryl-diyne-based Raman tag such as excellent photostability, negligible background interferences, and a linear dependence of the SRS signal on the TPP concentration, we successfully establish a quant. model to associate the mitochondrial membrane potential with the key pharmacokinetic parameters of TPP inside the live cells. The model reveals that reduction in the mitochondrial membrane potential leads to significant decreases in both the uptake rate and intracellular concentrations of TPP. Further, on the basis of the multiplexed SRS images concurrently highlighting the cellular proteins and lipids without further labeling, we find that the TPP uptake causes little cytotoxicity to the host cells. The bioorthogonal hyperspectral SRS microscopy imaging reveals that TPP can maintain stable affinity to mitochondria during the restructuring of mitochondrial networking, demonstrating its great potential for real-time monitoring of pharmacokinetics of small mols. associated with live biol. hosts, thereby promoting the development of mitochondria-targeting imaging probes and therapies in the near future.
Analytical Chemistry (Washington, DC, United States) published new progress about Bioorthogonal reaction (bioorthogonal hyperspectral SRS microscopy). 10602-04-7 belongs to class alcohols-buliding-blocks, and the molecular formula is C9H8O, HPLC of Formula: 10602-04-7.
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