Loznjak, Petra; Striegel, Lisa; Diaz De la Garza, Rocio I.; Rychlik, Michael; Jakobsen, Jette published the artcile< Quantification of folate in food using deconjugase of plant origin combined with LC-MS/MS: A method comparison of a large and diverse sample set>, Recommanded Product: Calcium folinate, the main research area is folate food plant glutamyl hydrolase LC MS; 10-Formylfolic acid (PubChem CID: 3080544); 5,10-Methenyltetrahydrofolate (PubChem CID: 439237); 5-Formyltetrahydrofolate (PubChem CID: 149436); 5-Methyltetrahydrofolate (PubChem CID: 439234); Deconjugase enzymes; Folates; Folic acid (PubChem CID: 6037); Food; LC-MS/MS; Method comparison; Microbiological assay; Tetrahydrofolate (PubChem CID: 1129).
A round robin comparison was performed in order to test the performance of a recently developed LC-MS/MS method for quantification of 6 folate forms. Eighty-nine samples representing the food groups of fruits, vegetables, legumes, cereals, dairy products, meat, and offal were analyzed by two LC-MS/MS methods and a microbiol. assay (MA). A plant-origin deconjugase enzyme (Arabidopsis thaliana) for deconjugation of folates (PE-LC-MS/MS), or animal-origin deconjugase (rat serum and chicken pancreas) (AE-LC-MS/MS) was used in the LC-MS/MS methods, each in a single enzymic step. In contrast, the MA involved tri-enzyme extraction including human plasma as a deconjugase. A significant bias of 17% lower and 25% higher results was found when PE-LC-MS/MS was compared to MA and AE-LC-MS/MS, resp. The PE-LC-MS/MS provides fast quantification of various folate vitamers and total folate content, which could be a proper substitute to the currently standardized but imprecise and time-consuming microbiol. assay in the future.
Food Chemistry published new progress about Allium cepa. 1492-18-8 belongs to class alcohols-buliding-blocks, and the molecular formula is C20H21CaN7O7, Recommanded Product: Calcium folinate.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts