《Self-Assembled Polyurethane Capsules with Selective Antimicrobial Activity against Gram-Negative E. coli》 was published in ACS Biomaterials Science & Engineering in 2020. These research results belong to Barman, Ranajit; Mondal, Tathagata; Sarkar, Jayita; Sikder, Amrita; Ghosh, Suhrit. SDS of cas: 534-03-2 The article mentions the following:
This article reports the antimicrobial activity of two segmented amphiphilic polyurethanes, PU-1 and PU-2, containing a primary or secondary amine group, resp. In acidic water, intrachain H-bonding among the urethanes followed by hierarchical assembly resulted in the formation of capsules (Dh = 120 ± 20 and 100 ± 17 nm for PU-1 and PU-2, resp.) with a highly pos. surface charge. They showed selective interactions with bacterial cell mimicking liposomes over mammalian cell mimicking liposomes with favorable enthalpy and entropy contributions, which was attributed to the electrostatic interaction and hydrophobic effect. Antimicrobial studies with Escherichia coli revealed very low min. inhibitory concentration (MIC) values of 7.8 and 15.6μg/mL for PU-1 and PU-2, resp., indicating their ability to efficiently kill Gram-neg. bacteria. Killing of Gram-pos. Staphylococcus aureus was noticed only at C = 500μg/mL, indicating unprecedented selectivity for E. coli, which was further confirmed by SEM (SEM) studies. Hemolysis assay revealed HC50 values of 453 and 847μg/mL for PU-1 and PU-2, resp., which were >50 times higher than their resp. MIC values, thus making them attractive antimicrobial materials. Ortho-nitrophenyl-β-galactoside (ONPG) assay and live-dead fluorescence assay confirmed that for both the polymers, a membrane disruption pathway was operative for wrapping of the bacterial membrane, similar to what was proposed for antimicrobial peptides. SEM images of polymer-treated E. coli bacteria helped in visualization of the pore formation and the disrupted membrane structure. In the experiment, the researchers used many compounds, for example, 2-Aminopropane-1,3-diol(cas: 534-03-2SDS of cas: 534-03-2)
2-Aminopropane-1,3-diol(cas: 534-03-2) belongs to anime. Primary amines having a tertiary alkyl group (R3CNH2) are difficult to prepare with most methods but are made industrially by the Ritter reaction. In this method a tertiary alcohol reacts with hydrogen cyanide (HCN) in the presence of a concentrated strong acid; a formamide, RNH―CHO, is formed first, which then undergoes hydrolysis.SDS of cas: 534-03-2
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