Wang, Libo; Li, Tengfei; Liu, Fangcheng; Liu, Dawei; Xu, Yaqin; Yang, Yu; Zhao, Yanbo; Wei, He published the artcile< Ultrasonic-assisted enzymatic extraction and characterization of polysaccharides from dandelion (Taraxacum officinale) leaves>, Computed Properties of 3458-28-4, the main research area is polysaccharide ultrasonic enzymic extraction leaf Taraxacum; Dandelion; Polysaccharides; Simulated digestion; Structural characterization; Ultrasonic-assisted enzymatic extraction.
Ultrasonic-assisted enzymic extraction (UAEE) technol. was employed to extract the polysaccharides from dandelion leaves (DLP). The extraction conditions were optimized by response surface method (RSM) and Box-Behnken design (BBD). The polysaccharide yield (14.05 ± 0.95%), which well matched with the predicted value of 14.27%, was obtained under the optimum parameters: ultrasound power 500 W, ultrasound time 42 min, cellulase amount 4.90%, and particle size 80 mesh. After purification by AB-8 Macroporous Resin and Sephadex G-100 column, a novel polysaccharide (DLP-I) was isolated and its mol. weight was estimated to be 87,000 g/mol by high performance liquid chromatog. (HPLC). Gas chromatog. (GC) anal. showed that DLP-I was composed of rhamnose, arabinose, mannose, galactose and glucose in the molar ratio of 1.14:1.00:1.05:4.76:1.52. 1D and 2D NMR spectra confirmed that the backbone of DLP-I was mainly consisted of →4)-α-D-Galp-(1→, →4)-β-D-Manp-(1→, →4)-α-D-Glcp-(1→, →2,4)-α-L-Rhap-(1→, and α-L-Araf-(1→ with branching at O-2 and O-4 of →2,4)-α-L-Rhap-(1→. In addition, the digestion results showed DLP-I could keep its stability during the simulated digestive juices.
International Journal of Biological Macromolecules published new progress about Food processing. 3458-28-4 belongs to class alcohols-buliding-blocks, and the molecular formula is C6H12O6, Computed Properties of 3458-28-4.
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