《Rhizobium rhizogenes mediated hairy root transformation and analysis of secondary metabolites in Phlogacanthus thyrsiflorus nees hairy roots using GC-MS》 was written by Singh, Shweta; Banerjee, Madhuparna; Kumar, Manish. Quality Control of 2-Aminopropane-1,3-diol And the article was included in Journal of Scientific & Industrial Research in 2020. The article conveys some information:
An efficient transformation system was developed for Phlogacanthus thyrsiflorus, a medicinal plant of northeast India, using Rhizobium rhizogenes strains A4 for infecting shoot tip, nodes and leaf explants derived from aseptically raised plants. The transformation frequency was found dependent on the explants as well as the co-culture periods. Highest transformation frequency of 95.69% was obtained from shoot tip explants co-cultured for 4 h. An average of 6.37 hairy roots per explant were obtained from shoot tip explants within three weeks of infection. Whereas, transformation frequency along with number of transformed roots were fewer in node and leaf explants. The presence of TL DNA of R. rhizogenes strains A4 in the hairy root genome of P. thyrsiflorus was confirmed through PCR based cloning and sequencing in which the sequencing results of the PCR amplicon confirmed the presence of rol b and rol c genes in the host genome. Sequence results for rol genes were found to be 97 percent and 99 percent similar with rol b and rol c gene present in NCBI database. GC-MS anal. of methanolic extract of the hairy roots reveals the presence of a few medicinally important secondary metabolites. In addition to this study using 2-Aminopropane-1,3-diol, there are many other studies that have used 2-Aminopropane-1,3-diol(cas: 534-03-2Quality Control of 2-Aminopropane-1,3-diol) was used in this study.
2-Aminopropane-1,3-diol(cas: 534-03-2) belongs to anime. The methylamines occur in small amounts in some plants. Many polyfunctional amines (i.e., those having other functional groups in the molecule) occur as alkaloids in plants—for example, mescaline, 2-(3,4,5-trimethoxyphenyl)ethylamine; the cyclic amines nicotine, atropine, morphine, and cocaine; and the quaternary salt choline, N-(2-hydroxyethyl)trimethylammonium chloride, which is present in nerve synapses and in plant and animal cells.Quality Control of 2-Aminopropane-1,3-diol
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts