Zou, Yuchen; Duan, Hanying; Li, Li; Chen, Xuju; Wang, Chao published the artcile< Quantification of polyglutamyl 5-methyltetrahydrofolate, monoglutamyl folate vitamers, and total folates in different berries and berry juice by UHPLC-MS/MS>, Formula: C20H21CaN7O7, the main research area is polyglutamyl methyltetrahydrofolate berry juice; 10-Formyldihydrofolate (PubChem CID: 168809); 10-Formylfolic acid (PubChem CID: 3080544); 10-Formyltetrahydrofolate (PubChem CID: 10); 5,10-Methenyltetrahydrofolate (PubChem CID: 439237); 5-Formyltetrahydrofolate (PubChem CID: 149436); 5-Methyltetrahydrofolate (PubChem CID: 439234); Berry; Deglutamylation; Folate vitamers; Folic acid (PubChem CID: 6037); Polyglutamyl 5-CH(3)THF; Recombinant γ-glutamyl hydrolase; Tetrahydrofolate (PubChem CID: 1129); Total folates.
Edible berries are good sources of several phytochems. However, the native folate levels in berries are not well known. The structure of native folates contains polyglutamyl chains, which reportedly jeopardize the bioavailability of native folates; further γ-glutamyl hydrolase (GGH) can deglutamylate polyglutamyl chains. In this study we use a validated ultra-high performance liquid chromatog.-tandem mass spectrometry (UHPLC-MS/MS) method to determine the distribution of polyglutamyl 5-methyltetrahydrofolate (5-CH3THF-Glun), folate vitamers, and total folates in different berries and to monitor changes in their concentration during processing of berries into juice. The pre-boiling treatment was optimized during extraction to stabilize the native polyglutamyl folates profile and facilitate folate extraction, which can replace the traditional di-enzyme treatment process. Addnl., the efficiency of com. available human recombinant GGH was tested and it was found that a 10 μg GGH/mL extract at a pH of 6 could completely deconjugate polyglutamyl folates into monoglutamyl folates when incubated for 30 min. Pure human recombinant GGH with a higher catalytic efficiency and stable enzymic properties was better than traditional folate conjugase for this purpose. From exptl. anal., it could be inferred that strawberries and blackberries contained the highest amount of total folates (93-118 μg/100 g), while the total folate contents in blueberries were the lowest. Most of the investigated berries are good to excellent folate sources. This study is the first time that 5-CH3THF-Glun and distribution of folate vitamers in various berries are quantitated. Further, this is the first study to show the application of recombinant pure GGH for the deconjugation of polyglutamyl folates for folate vitamers and total folates anal.
Food Chemistry published new progress about Berry juice. 1492-18-8 belongs to class alcohols-buliding-blocks, and the molecular formula is C20H21CaN7O7, Formula: C20H21CaN7O7.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts