Waldie, Sarah; Moulin, Martine; Porcar, Lionel; Pichler, Harald; Strohmeier, Gernot A.; Skoda, Maximilian; Forsyth, V. Trevor; Haertlein, Michael; Maric, Selma; Cardenas, Marite published the artcile< The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions>, SDS of cas: 434-16-2, the main research area is cholesterol lipid bilayer small angle neutron scattering MS NMR.
The deuteration of biomols. provides advanced opportunities for neutron scattering studies. For low resolution studies using techniques such as small-angle neutron scattering and neutron reflection, the level of deuteration of a sample can be varied to match the scattering length d. of a specific D2O/H2O solvent mixture This can be of major value in structural studies where specific regions of a complex system can be highlighted, and others rendered invisible. This is especially useful in analyses of the structure and dynamics of membrane components. In mammalian membranes, the presence of cholesterol is crucial in modulating the properties of lipids and in their interaction with proteins. Here, a protocol is described for the production of partially deuterated cholesterol which has a neutron scattering length d. that matches that of 100% D2O solvent (hereby named matchout cholesterol). The level of deuteration was determined by mass spectrometry and NMR. The cholesterol match-point was verified exptl. using small angle neutron scattering. The matchout cholesterol was used to investigate the incorporation of cholesterol in various phosphatidylcholine supported lipid bilayers by neutron reflectometry. The study included both saturated and unsaturated lipids, as well as lipids with varying chain lengths. It was found that cholesterol is distributed asym. within the bilayer, positioned closer to the headgroups of the lipids than to the middle of the tail core, regardless of the phosphatidylcholine species.
Scientific Reports published new progress about Bilayer membranes. 434-16-2 belongs to class alcohols-buliding-blocks, and the molecular formula is C27H44O, SDS of cas: 434-16-2.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts