Lam, Albert T.; Zhang, Xiao-Nan; Courouble, Valentine V.; Strutzenberg, Timothy S.; Pei, Hua; Stiles, Bangyan L.; Louie, Stan G.; Griffin, Patrick R.; Zhang, Yong published the artcile< A Bifunctional NAD+ for Profiling Poly-ADP-Ribosylation-Dependent Interacting Proteins>, Recommanded Product: 2-(3-Methyl-3H-diazirin-3-yl)ethanol, the main research area is poly ADP ribosylation bifunctional fluorescent ligand protein interaction.
Protein poly-ADP-ribosylation (PARylation) is a heterogeneous and dynamic post-translational modification regulated by various writers, readers, and erasers. It participates in a variety of biol. events and is involved in many human diseases. Currently, tools and technologies have yet to be developed for unambiguously defining readers and erasers of individual PARylated proteins or cognate PARylated proteins for known readers and erasers. Here, we report the generation of a bifunctional NAD (NAD+) characterized by diazirine-modified adenine and clickable ribose. By serving as an excellent substrate for poly-ADP-ribose polymerase 1 (PARP1)-catalyzed PARylation, the generated bifunctional NAD+ enables photo-crosslinking and enrichment of PARylation-dependent interacting proteins for proteomic identification. This bifunctional NAD+ provides an important tool for mapping cellular interaction networks centered on protein PARylation, which are essential for elucidating the roles of PARylation-based signals or activities in physiol. and pathophysiol. processes.
ACS Chemical Biology published new progress about Apoptosis. 25055-82-7 belongs to class alcohols-buliding-blocks, and the molecular formula is C4H8N2O, Recommanded Product: 2-(3-Methyl-3H-diazirin-3-yl)ethanol.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts