Yu, Li; Bhattacharya, Dhruva; Wang, Zhenhan; Wang, Mingwu published an article in Graefe’s Archive for Clinical and Experimental Ophthalmology. The title of the article was 《Topical administration of ambroxol eye drops augments tear secretion in rabbits》.Synthetic Route of C13H19Br2ClN2O The author mentioned the following in the article:
To investigate if topical administration of ambroxol promotes tear secretion and to compare with Diquas ophthalmic eye drop. Two consecutive studies were conducted using sixteen (32 eyes) New Zealand White rabbits. The first study compared the efficacy of ambroxol hydrochloride (0.05%, 0.2%, and 1.0%) on tear and mucin secretion when administered twice daily. Tear secretion was assessed by Schirmer test I and mucin production by conjunctival impression cytol. and PAS stain. The second study compared 0.2% ambroxol hydrochloride with Diquas. A human goblet cell line and human conjunctival tissue were used to test the effect of ambroxol hydrochloride on the expression of aquaporin 5 (AQP5) and MUC5AC, using reverse transcriptase-quant. polymerase chain reaction and immunoblotting. All three concentrations of ambroxol hydrochloride demonstrated significant efficacy on tear stimulation within 2 wk of treatment and total mucin component appeared increased. When administered topically twice daily, 0.2% ambroxol hydrochloride was more effective in augmenting tear secretion than Diquas. With 24 h of treatment, 5μM of ambroxol hydrochloride upregulated AQP5 and MU5AC mRNA and MUC5AC protein in a goblet cell line. When tested on preserved human conjunctiva tissue, a trend of increased production of MUC5AC protein was seen (P = 0.26). Ambroxol is effective in augmenting tear secretion at the ocular surface in rabbits. With actions desirable of a candidate dry eye drug, further investigation of ambroxol and related compounds is warranted to explore their value toward clin. application. The experimental part of the paper was very detailed, including the reaction process of trans-4-((2-Amino-3,5-dibromobenzyl)amino)cyclohexanol hydrochloride(cas: 23828-92-4Synthetic Route of C13H19Br2ClN2O)
trans-4-((2-Amino-3,5-dibromobenzyl)amino)cyclohexanol hydrochloride(cas: 23828-92-4) is a mucolytic expectorant and a metabolite of bromhexine.Synthetic Route of C13H19Br2ClN2O It is used in the treatment of respiratory disorders characterized by viscous or excessive mucus.
Referemce:
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Alcohols – Chemistry LibreTexts