Luo, Yitao; Zhang, Chengqiang; Ma, Li; Zhang, Yuxiao; Liu, Zhengyuan; Chen, Li; Wang, Rui; Luan, Yujing; Rao, Yulan published the artcile< Measurement of 7-dehydrocholesterol and cholesterol in hair can be used in the diagnosis of Smith-Lemli-Opitz syndrome>, COA of Formula: C27H44O, the main research area is Smith Lemli Opitz syndrome hair diagnosis 7 dehydrocholesterol cholesterol; diagnostic test; gas chromatography-mass spectrometry; hair analysis; micro-pulverized extraction; microwave-assisted derivatization.
7-Dehydrocholesterol (7-DHC) and cholesterol (CHOL) are biomarkers of Smith-Lemli-Opitz Syndrome (SLOS), a congenital autosomal recessive disorder characterized by elevated 7-DHC level in patients. Hair samples have been shown to have great diagnostic and research value, which has long been neglected in the SLOS field. In this study, we sought to investigate the feasibility of using hair for SLOS diagnosis. In the presence of antioxidants (2,6-ditert-butyl-4-methylphenol and triphenylphosphine), hair samples were completely pulverized and extracted by micro-pulverized extraction in alk. solution or in n-hexane. After microwave-assisted derivatization with N,O-Bis(trimethylsilyl)trifluoroacetamide, the analytes were measured by GC-MS. We found that the limits of determination for 7-DHC and CHOL were 10 ng/mg and 8 ng/mg, resp. In addition, good linearity was obtained in the range of 50-4000 ng/mg and 30-6000 ng/mg for 7-DHC and CHOL, resp., which fully meets the requirement for SLOS diagnosis and related research. Finally, by applying the proposed method to real hair samples collected from 14 healthy infants and two suspected SLOS patients, we confirmed the feasibility of hair anal. as a diagnostic tool for SLOS. In conclusion, we present an optimized and validated anal. method for the simultaneous determination of two SLOS biomarkers using human hair.
Journal of Lipid Research published new progress about Antioxidants. 434-16-2 belongs to class alcohols-buliding-blocks, and the molecular formula is C27H44O, COA of Formula: C27H44O.
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