Haykal, Tony; Younes, Maria; El Khoury, Marianne; Ammoury, Carl; Tannous, Stephanie; Hodroj, Mohammad H.; Sarkis, Rita; Gasilova, Natalia; Menin, Laure; Rizk, Sandra published the artcile< The pro-apoptotic properties of a phytonutrient rich infusion of A. cherimola leaf extract on AML cells>, COA of Formula: C6H10O8, the main research area is Annona cherimola leaf extract proapoptotic phytonutrient acute myeloid leukemia; Acute Myeloid Leukemia; Annona cherimola tea infusion; Anti-oxidant; Apoptosis; Phytochemicals.
Annonaceae family has broad uses in herbal medicine for treatment of several diseases, whether through seeds′ or leaves′ extracts The present study investigates the antiproliferative and antitumor activity of Annona cherimola aqueous leaf (AAL) extract/infusion in acute myeloid leukemia (AML) cell lines in vitro. High-resolution LC-MS was first used to analyze the composition of the aqueous extract Cell proliferation assay, Annexin V staining, cell cycle anal., dual Annexin V/PI staining, cell death quantification by ELISA, ROS level detection and Western Blotting were then performed to elucidate the therapeutic effects of AAL extract The results obtained revealed a potent antioxidant activity of AAL extract Moreover, the extract exhibited dose- and time-dependent antiproliferative effects on AML cell lines by decreasing cell viability with an IC50 of 5.03% (volume/volume) at 24 h of treatment of KG-1 cells. This decrease in viability was accompanied with a significant increase in apoptotic cell death with cell cycle arrest and flipping of the phosphatidylserine from the inner to the outer leaflet of the cell membrane. The resp. overexpression and downregulation of proapoptotic proteins like cleaved caspase-8, cleaved PARP-1 and Bax and antiapoptotic proteins like Bcl-2 further validated the apoptotic pathway induced by AAL on AML cells. Finally, LC-MS revealed the presence of several compounds like fatty acids, terpenes, phenolics, cinnamic acids and flavonoids that could contribute to the antioxidant and anti-cancer effects of this herbal infusion. In addition to the generally known nutritional effects of the Annona cherimola fruit and leaves, the presented data validates the antioxidant and anti-cancerous effects of the leaf infusion on AML cell lines, proposing its potential therapeutic use against acute myeloid leukemia with future in vivo and clin. trials.
Biomedicine & Pharmacotherapy published new progress about Acute myeloid leukemia (cell lines). 87-73-0 belongs to class alcohols-buliding-blocks, and the molecular formula is C6H10O8, COA of Formula: C6H10O8.
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