Ziemianowicz, Daniel S.; MacCallum, Justin L.; Schriemer, David C. published the artcile< Correlation between Labeling Yield and Surface Accessibility in Covalent Labeling Mass Spectrometry>, Synthetic Route of 25055-82-7, the main research area is surface accessibility covalent labeling mass spectrometry.
The functional properties of a protein are strongly influenced by its topog., or the solvent-facing contour map of its surface. Together with crosslinking, covalent labeling mass spectrometry (CL-MS) has the potential to contribute topog. data through the measurement of surface accessibility. However, recent efforts to correlate measures of surface accessibility with labeling yield have been met with mixed success. Most applications of CL-MS involve differential anal. of protein interactions (i.e., footprinting experiments) where such inconsistencies have limited effect. Extending CL-MS into structural anal. requires an improved evaluation of the relation between labeling and surface exposure. The authors applied recently developed diazirine reagents to obtain deep coverage of the large motor domain of Eg5 (a mitotic kinesin), and together with computational methods the authors correlated labeling yields with accessibility data in a number of ways. Correlations can indeed be seen at a local structural level, but these correlations do not extend across the structure. The lack of correlation arises from the influence of protein dynamics and chem. composition on reagent partitioning and, thus, also on labeling yield. The authors’ use of CL-MS data should be considered in light of “”chem. accessibility”” rather than “”solvent accessibility”” and suggest that CL-MS data would be a useful tool in the fundamental study of protein-solute interactions.
Journal of the American Society for Mass Spectrometry published new progress about Kinesin-like protein KIF11 Role: ANT (Analyte), BSU (Biological Study, Unclassified), ANST (Analytical Study), BIOL (Biological Study) (Eg5, ADP-bound). 25055-82-7 belongs to class alcohols-buliding-blocks, and the molecular formula is C4H8N2O, Synthetic Route of 25055-82-7.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts