Duijndam, Britt;Tedeschi, Massimo;van der Stel, Wanda;van den Hoorn, Tineke;van der Burg, Bart;Bouwman, Peter;van der Laan, Jan Willem;van de Water, Bob published 《Evaluation of an imaging-based in vitro screening platform for estrogenic activity with OECD reference chemicals》 in 2022. The article was appeared in 《Toxicology In Vitro》. They have made some progress in their research.Category: alcohols-buliding-blocks The article mentions the following:
Estrogen receptor alpha (ERα) is often a primary target of endocrine disrupting chems. (EDCs) and therefore several biochem. and cell-based assays for the detection of chems. with estrogenic properties have been developed in the past. However, the current approaches are not suitable for the monitoring of pathway activation dynamics, and they are mostly based on expression constructs that lack physiol. promoter regulation. We recently developed MCF7 fluorescent reporter cell lines of 3 different green fluorescent protein (GFP)-tagged ERα target genes: GREB1, PGR and TFF1. These reporters are under control of the full physiol. promoter region and allow the monitoring of dynamic pro-proliferative pathway activation on a single cell level using a live-cell imaging set-up. In this study, we systematically characterized the response of these reporters to a full reference compound set of known estrogenic and non-estrogenic chems. as defined by the Organization for Economic Co-Operation and Development (OECD). We linked activation of the pro-proliferative ERα pathway to a potential adverse outcome by addnl. monitoring cell cycle progression and proliferation. The correct classification of the OECD reference compounds showed that our reporter platform has the same sensitivity and specificity as other validated artificial ERα pathway reporters, such as the ERα CALUX and VM7 Luc ER TA assay. By monitoring several key events (i.e. ER target activation, cell cycle progression and proliferation), and subsequently determining Point-of-Departure (POD) values, our reporter panel can be used in high-throughput testing for a physiol. more relevant, quant. temporal endocrine modulation anal. to improve human carcinogen risk assessment. To complete the study, the researchers used 4-tert-Octylphenol (cas: 140-66-9) .
4-tert-Octylphenol(cas: 140-66-9) has been used as a reference standard for the determination of the analyte in water samples using ultra-high-performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS).Category: alcohols-buliding-blocks
Reference:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts