Reeves, Richard E.;Meleney, Henry E.;Frye, William W. published 《Cultivation of Entamoeba histolytica with penicillin-inhibited Bacteroides symbiosus cells. I. Pyridoxine requirement》. The research results were published in《American Journal of Hygiene》 in 1959.SDS of cas: 148-51-6 The article conveys some information:
In a modified Shaffer-Frye culture system it was found that the multiplication of Entamoeba histolytica is strongly inhibited by low concentrations of deoxypyridoxol. The effect of this substance is reversed by the addition of pyridoxal, pyridoxylamine, pyridoxol or pyridoxal phosphate. The last substance was shown to be more effective than pyridoxol in reversing the action of desoxypyridoxol. Conditions were found which allowed the determination of the concentrations of desoxypyridoxol required to reduce to half-maximum the multiplication of E. histolytica. These half-maximum concentrations were reproducible for given stains of amebae, but significant differences were found among 5 strains examined. The F-22 and a newly isolated strain (JH) were more sensitive, the DKB, 200 and K-9 strains were less sensitive to the anti-metabolite. Neither the F-22 nor the DKB strain developed the ability to tolerate larger amounts of anti-metabolite upon continued cultivation in media containing it. Desoxypyridoxol was also effective in preventing the growth of E. histolytica in Cleveland-Collier cultures in the presence of a multiplying mixed-bacterial flora. These results show that there is a pyriodoxine requirement for the multiplication of E. histolytica in the MS-F system. It is not definitely established whether the action of the anti-metabolite is directly on the ameba or upon some phase of the residual metabolism of the accompanying penicillin-inhibited bacterial cells.5-(hydroxymethyl)-2,4-dimethylpyridin-3-ol hydrochloride (cas: 148-51-6) were involved in the experimental procedure.
5-(hydroxymethyl)-2,4-dimethylpyridin-3-ol hydrochloride(cas:148-51-6 SDS of cas: 148-51-6) is a vitamin B6 antimetabolite with diverse biological activities. It inhibits transport of pyridoxine , pyridoxal, and pyridoxamine in and reduces growth of S. carlsbergensis cells. DOP inhibits sphingosine-1-phosphate (S1P) lyase and reduces cyclic stretch-induced apoptosis in alveolar epithelial MLE-12 cells.
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