Lee, Seok-Hee;Seo, Huiwon;Seo, Hyeyeong;Lazari, Maria;D’Agostino, Martin;Byrd, Nick;Yoon, Kyong Sup;Lee, Hee-Seok;Park, Yooheon published 《An In vitro dimerization assay for the adverse outcome pathway approach in risk assessment of human estrogen receptor α-mediated endocrine-disrupting chemicals》. The research results were published in《Chemosphere》 in 2022.Product Details of 140-66-9 The article conveys some information:
The adverse outcome pathway (AOP) has been recently proposed as an effective framework for chem. risk assessment. The AOP framework offers the advantage of effectively integrating individual in vitro studies and in silico prediction models. Thus, the development of an effective testing method to measure key events caused by chems. is essential for chem. risk assessment through a fully developed AOP framework. We developed a human cell-based estrogen receptor α (ERα) dimerization assay using the bioluminescence resonance energy transfer (BRET) technique and evaluated the ERα dimerization activities of 72 chems. Fifty-one chems. were identified to mediate dimerization of ERα, and the BRET-based ERα dimerization assay could effectively measure the events that mediated dimerization of ERα by the estrogenic chems. These results were compared with the results of pre-existing assay to determine whether the BRET-based ERα dimerization assay could be employed as an in vitro test method to provide scientific information for explaining key events as a part of the AOP framework. Consequently, we propose that the BRET-based ERα dimerization assay is suitable for measuring the chem.-mediated dimerization of ERα, a key event in the AOP framework for cellular-level risk assessment of estrogenic chems.4-tert-Octylphenol (cas: 140-66-9) were involved in the experimental procedure.
4-tert-Octylphenol(cas: 140-66-9) is a common environmental pollutant showing weak estrogenic effects.Product Details of 140-66-9 It has been shown to cause harm to vertebrate male reproductive systems.
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