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The expression profiles of acid-stable alpha-amylase and acid-labile alpha-amylase of Aspergillus luchuensis mut. Kawachii effect on the microstructure of koji and alcohol fermentation
We investigated the contribution of the complex expressions of two alpha-amylases, namely acid-stable alpha-amylase (AS-amylase) and acid-labile alpha-amylase (AL-amylase), from Aspergillus kawachii to the microstructure of koji and the brewing. AL-amylase was found to be the primary contributor to the decomposition of starch in the early stage of koji making. From the middle stage, both alpha-amylases decomposed the starch in a coordinated manner, and at the final stage, acid-stable alpha-amylase and glucoamylase decomposed the starch granules. Characterization of koji prepared by the single disruptions of AS-amylase or AL-amylase genes, double disruption of both alpha-amylase genes, and triple disruption of two alpha-amylase and glucoamylase genes in A. kawachii cells indicated that both alpha-amylases can work in a synergistic manner to decompose starches during koji making. AL-amylase was found, for the first time, to play an important role in starch decomposition in koji. The speed of alcohol fermentation and ester contents of the fermented mash were higher the mash prepared the control strain, followed by single, double, and triple disruptants. These results indicate that the microstructure of koji plays a role in promoting alcohol fermentation and flavor development.
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Reference:
Alcohol – Wikipedia,
,Alcohols – Chemistry LibreTexts